Evans, Brown and Robson start for Quins
England's loss is Quins' gain as director of rugby Conor O'Shea has confirmed full back Mike Brown and George Robson will start against Gloucester tomorrow evening.
The pair were released from head coach Stuart Lancaster's squad mid-week, along with Jordan Turner-Hall, who will be rested for the weekend as he nurses a knee problem.
Tom Casson continues to partner Matt Hopper in the centre, while fly half Nick Evans, who agreed a three-year deal at the Stoop this week, makes his comeback from an ankle injury.
Hooker Joe Gray will be out to impress with England short of options at number two for the forthcoming autumn internationals, as he too comes back from injury.
Luke Wallace takes skipper Chris Robshaws' place in the back row, alongside Maurie Fa'asavalu and captain Nick Easter.
Harlequins team: 15. Mike Brown, 14. Tom Williams, 13. Matt Hopper, 12. Tom Casson, 11. Sam Smith, 10. Nick Evans, 9. Karl Dickson; 1. Mark Lambert, 2. Joe Gray, 3. James Johnston, 4. Olly Kohn, 5. George Robson, 6. Maurie Fa’asavalu, 7. Luke Wallace, 8. Nick Easter (C).
Replacements: 16. Rob Buchanan, 17. Darryl Marfo, 18. Will Collier, 19. Charlie Matthews, 20. Tom Guest, 21. Jordan Burns, 22. Ben Botica, 23. Seb Stegmann.